Equipe "Dissection optogénétique des circuits spinaux sous-tendant la locomotion"
Marion-Anne is a neuroscience master student currently completing her last year at the Ecole Normale Supérieure (ENS) de Cachan - Paris Saclay and Université Pierre et Marie Curie (Paris, France). She developed her fascination for neuroscience during two previous experiences: first in 2014 she learned brain slice electrophysiology basics at Université Paris Descartes (Paris) in the team headed by Vivien Chevaleyre and Rebecca Piskorowski, where she studied the effects of two neuropeptides on the inhibitory transmission in area CA2 by performing extracellular recordings. Then she started a 10-month internship in György Buzsáki’s laboratory at New York University Neuroscience Institute in October 2015 to learn the most advanced techniques in freely moving neurophysiology and systems neuroscience in rodents. To unravel the fundamental circuit properties that govern the generation of a robust and reliable head-direction signal, they combined electrode recording and optogenetic manipulation to probe the functional connectivity of the head-direction neurons circuit and its internal dynamics. She joined Claire Wyart’s laboratory in 2016 for her master degree since she wants to discover new technics to study neuronal activity in vivo.
Recently, cells strategically positioned between cerebrospinal fluid (CSF) and neuronal parenchyma in the ventral spinal cord are thought to constitute intraspinal GABAergic sensory neurons that could play a role of interface integrating CSF signals and thus modulating locomotion. Marion-Anne will take advantage of the zebrafish model to unveil in vivo spinal CSF-cNs functional activity during changes of the pH in the central canal, and study the involvement of pH sensitive channels in this response. Thanks to transgenic larvae’s transparent body, she will be able to monitor and modify pH in the central canal of the living animal while recording calcium transients in spinal CSF-cNs with a two-photon microscope, and performing fluorescent-targeted patch clamp of these cells in different zebrafish lines.