iGenSeq : genotyping and sequencing core facility

Open / close summary
iGenSeq provides tools and services for genome analysis to academics or industrials. More specifically, services include high-throuput sequencing, real-time PCR and animals genotyping,  as well as purification and analysis of nucleic acids. Each project submitted to the core facility is discussed with the investigator to optimize the design and the feasibility. This platform masters the techniques of highthroughput sequencing and can also provide medium-throughput new generation sequencing and associated biostatistics services. iGenSeq is part of the ICM core facility network (vectorology, histology, microscopy, cellular culture…) which facilitates translational research projects.

ACTIVITIES

• High-Throughput using Illumina system including Exome, RNAseq (including single cell, mRNAseq, total RNAseq, 3′ RNAseq), MethylSeq (Capture or whole genome bisulfite), miRNAseq, Genome, chipset and custom capture panel.
• Real-time PCR: SybrGreen or probespecific technology, RNA quantification, Droplet Digital PCR, HRM
• Sequencing: medium-throughput using GS Junior and Miseq Illumina systems including amplicon sequencing, long-range, ultra-deep sequencing applications and Capture (DNA and RNA)
• Genotyping: animals (tissue)
• PCR products purification and analysis: PCR or sequence purification and quantitative analysis of DNA or RNA

EQUIPMENT

• 3 Real-time PCR (1×96 wells, 384 wells, 1536 wells)
• 11 PCR apparatus (Applied Biosystem, MJ Research, Eppendorf)
• 1 Miseq Illumina system
• 1 Bravo pipetting system
• 1 plateLoc system
• 2 Labchip GX
• 2 NextSeq Illumina System
• 1 Novaseq6000 Illumina System
• 1 TapeStation Agilent System
• 1 Bioruptor Diagenode System

TEAM

Stéphanie Baulac (Scientific coordinator)
Yannick Marie (Operational coordinator)
Jérôme Garrigue (Animal genotyping)
Bruno Choisi  (Animal Genotyping)
Delphine Bouteiller (Real-time PCR and ISO9001)
Emeline Mundwiller (Sequencing)
Claire Sophie Davoine (Sequencing )
Agnès Rastetter (Sequencing)

CERTIFICATION

certification

 

 

 

 

 

CONTRACT RESEARCH SERVICES DELIVERED BY THE VECTOROLOGY PLATFORM AND GENOTYPING-SEQUENCING PLATFORM.

CONTACT

igenseq@icm-institute.org