CELIS Cutting-edge equipment for high performance cell culture

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This core facility makes available a large panel of cell and tissue culture models and up-to-date equipment to academic researchers, biotech companies and industrials that pursue experimental projects on pathologies of the brain and spinal cord. Specific activities operated by CELIS staff include, automated and conventional fluorescence imaging, automated cell culture, electrophysiological recordings and induced pluripotent stem (iPS) cell production/characterization. The facility is part of the ICM core facility network and supported by “Investissements d’avenir” IHU-A-ICM and NeurATRIS.

ACTIVITIES

• Modeling brain, spinal cord and skeletal muscle disorders with cell or tissue culture systems
• Molecular and cellular pharmacology; Medium throughput screening
• Conventional or automated fluorescent imaging; infrared fluorescence imaging
• High throughput fluorescence imaging; Functional imaging; Videomicroscopy
• Near infrared or chemiluminescence imaging for blot detection
• High performance electrochemiluminescence immunoassays
• Flow cytometry for cell analysis
• Electrophysiological characterization of ion channel dysfunction (CELIS-ePhyS)
• Production and characterization of iPS cell lines (CELIS-iPS)

EQUIPMENT

• 18 cell culture boxes (L2 confinement) with CO2 incubators and safety cabinets
• 2 automated workstations for high throughput imaging (Arrayscan XTi, CellInsight)
• Database and application server with remote PC workstations for post-hoc image analysis
• 5 inverted fluorescent microscopes (3 Zeiss Axio Observer7; 1 Nikon Ti Eclipse; 1 NikonTE 2000) for functional imaging, videomicroscopy or optical sectioning (ApoTome)
• Bright field and fluorescence stereomicroscopes
• Odyssey and ChemiDoc Touch imaging systems for blot detection
• MESO QuickPlex SQ 120 for multiplex immunoassays
• BD FACSVerse flow cytometer; automated cell counters (Tali, Countess)
• 2D and 4D Nucleofector systems
• Workstation for automated cell culture
• 1 box for the generation of iPS cell lines
• 1 box for manipulating radiochemicals
• Set-ups for electrophysiological recordings on isolated cells, zebrafish or brain slices

TEAM

Patrick-Pierre Michel — Scientific manager
Laetitia Strehl — Operational manager
David Akbar – Engineer, Cell culture imaging
Jérôme Garrigue – Engineer, Automated cell culture
Stéphanie Godard-Bauche – Engineer, Operational support
Carine Dalle —Research engineer, electrophysiology
Charlotte Deleuze – Research engineer,
Stéphanie Bigou — Engineer, IPS cell production

CONTACT

celis@icm-institute.org